Micro-irradiation tools to visualize base excision repair and single-strand break repair
نویسندگان
چکیده
منابع مشابه
Evolving DNA Repair Polymerases: From Double—Strand Break Repair to Base Excision Repair and VDJ Recombination
Currently five polymerases have been identified in Escherichia coli, at least eight in Saccharo‐ myces cerevisiae, nine in Schizosaccharomyces pombe, and fourteen in humans [1-4]. Based on the primary structure of the catalytic subunits, DNA polymerases have been classified into different families. Eukaryotic organisms have four families: A family (Polγ, Polθ and Polν), B family (Polα, Polδ, Po...
متن کاملDistinct spatiotemporal patterns and PARP dependence of XRCC1 recruitment to single-strand break and base excision repair
Single-strand break repair (SSBR) and base excision repair (BER) of modified bases and abasic sites share several players. Among them is XRCC1, an essential scaffold protein with no enzymatic activity, required for the coordination of both pathways. XRCC1 is recruited to SSBR by PARP-1, responsible for the initial recognition of the break. The recruitment of XRCC1 to BER is still poorly underst...
متن کاملDouble strand break repair.
DNA double-strand breaks (DSBs) are the most dangerous form of DNA damage and can lead to death, mutation, or malignant transformation. Mammalian cells use three major pathways to repair DSBs: homologous recombination (HR), classical nonhomologous end joining (C-NHEJ), and alternative end joining (A-NHEJ). Cells choose among the pathways by interactions of the pathways with CtIP and 53BP1. HR i...
متن کاملInvolvement of Nucleotide Excision and Mismatch Repair Mechanisms in Double Strand Break Repair
Living organisms are constantly threatened by environmental DNA-damaging agents, including UV and ionizing radiation (IR). Repair of various forms of DNA damage caused by IR is normally thought to follow lesion-specific repair pathways with distinct enzymatic machinery. DNA double strand break is one of the most serious kinds of damage induced by IR, which is repaired through double strand brea...
متن کاملApplication of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
Highly coordinated DNA repair pathways exist to detect, excise and replace damaged DNA bases, and coordinate repair of DNA strand breaks. While molecular biology techniques have clarified structure, enzymatic functions, and kinetics of repair proteins, there is still a need to understand how repair is coordinated within the nucleus. Laser micro-irradiation offers a powerful tool for inducing DN...
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ژورنال
عنوان ژورنال: DNA Repair
سال: 2015
ISSN: 1568-7864
DOI: 10.1016/j.dnarep.2015.05.001